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Water Quality and Algae Surveys Data

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Near-Surface Water-Quality and Algae Surveys along the Caloosahatchee River, Florida, USA, June 2016


Algae samples were taken from the water-surface by dipping a 1-liter bottle, leaving 1 inch of air space at the top, iced on site and shipped overnight to the laboratory. Samples were observed and photographed using differential interference contrast (DIC) microscopy with an Olympus BX51 research microscope at 200x or 400x magnification. Algae organisms were identified according to Rosen and St. Amand (2015).

Moving-boat surveys are a useful tool to produce water-quality maps of aquatic systems, covering large areas during a short period of time (Booth and others, 2014, Soderqvist and Patino, 2010, Byrne and Gabaldon, 2008). On June 1, 2016, a water-quality survey was completed along the freshwater reach of the Caloosahatchee River, from Lake Okeechobee at Moore Haven Lock and Dam (S-77) to W. P. Franklin Lock and Dam (S-79). Sensor and coordinate data were recorded as the boat traveled through the study area, making measurements using an onboard flow-through system. Water was collected using a brass thru-hull fitting on the underside of the boat, at approximately 1 ft depth. Specific conductance, temperature, dissolved oxygen, pH, and turbidity data were recorded at 5-second intervals using a YSI EXO, while an optical nitrate sensor (Satlantic SUNA V2) was used to collect data on 1-minute intervals. Sensors were calibrated prior to the survey following procedures described in Wagner and others (2006) and Pellerin and others (2013). In addition to sensor data, discrete water samples were collected at 13 locations for nitrate+nitrite laboratory analyses and algae identification. Optical nitrate sensor data were compared to laboratory results to determine possible instrument bias and necessary calibration. Techniques and methods used are described by Pellerin and others (2013). The resultant calibration rating was used to adjust the sensor data and produce a corrected dataset of nitrate+nitrite, as nitrogen for the map presented.

References cited:

Booth, Amanda C., Knight, Travis M., and Soderqvist, Lars E., 2014, Near-Surface Water-Quality Surveys of the Caloosahatchee River and Downstream Estuaries, Florida, USA: U.S. Geological Survey Data Release, http://dx.doi.org/10.5066/F70863BC.

Byrne, M.J., Gabaldon, J.N., 2008, Hydrodynamic Characteristics and Salinity Patterns in Estero Bay, Lee County, Florida: U.S. Geological Survey Scientific Investigations Report 2007-5217, 33 p., http://pubs.usgs.gov/sir/2007/5217/.

Pellerin, B.A., Bergamaschi, B.A., Downing, B.D., Franco Saraceno, J., Garrett, J.D., and Olsen, L.D., 2013, Optical techniques for the determination of nitrate in environmental waters: guidelines for instrument selection, operation, deployment, maintenance, quality assurance, and data reporting: U.S. Geological Survey Techniques and Methods 1-D5, https://pubs.er.usgs.gov/publication/tm1D5.

Rosen, B.H., and St. Amand, Ann, Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native Communities: U.S. Geological Survey Open-File Report 2015-1164, 44 p., http://dx.doi.org/10.3133/ofr20151164.

Soderqvist, L.E., and Patino, Eduardo, 2010, Seasonal and spatial distribution of freshwater flow and salinity in the Ten Thousand Islands Estuary, Florida, 2007-2009: U.S. Geological Survey Data Series 501, 24 p. http://pubs.usgs.gov/ds/501/.

Wagner, R.J., Boulger, Jr., R.W., Oblinger, C.J., and Smith, B.A. 2006, Guidelines and standard procedures for continuous water-quality monitors: Station operation, record computation, and data reporting: U.S. Geological Survey Techniques and Methods 1-D3, 51 p. http://pubs.usgs.gov/tm/2006/tm1D3/.

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