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Preliminary Characterization of the Microbial Ecology of the Upper Florida Aquifer and Literature Review of the Microbe-Induced Changes in Metal Mobility and Toxicity during Aquifer Storage and Recovery Testing

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Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: John Lisle
Publication_Date: 2005
Title:
Preliminary Characterization of the Microbial Ecology of the Upper Florida Aquifer and Literature Review of the Microbe-Induced Changes in Metal Mobility and Toxicity during Aquifer Storage and Recovery Testing
Geospatial_Data_Presentation_Form: project
Online_Linkage: https://sofia.usgs.gov/projects/index.php?project_url=microbe
Description:
Abstract:
Recently, as part of the Comprehensive Everglades Restoration Plan Aquifer Storage and Recovery (ASR) Regional Study project, the project delivery team has proposed that microbiological studies be performed during the different phases of ASR well field planning, development, and use. The focus of ongoing research in ASR microbiology is from the perspective of public health and these efforts are designed to address regulatory issues, such as survival of indicator microorganisms and pathogens.

The scope of work includes a suite of assays that will compliment the ongoing public health microbiology investigations and address concerns about the impact of injected waters on the native microbial ecology and biogeochemistry. Additionally, a literature review will be conducted to compile, synthesize and assess the published literature on microbially induced changes in mobility and toxicity of metals associated with aquifer matrices in geologic formations that have been designated as storage zones for ASR systems located in South Florida.
Purpose:
This project will address concerns about the impact of injected waters on the native microbial ecology and biogeochemistry and will conduct a literature review to compile, synthesize and assess the published literature.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 2003
Ending_Date: 2004
Currentness_Reference: publication date
Status:
Progress: Complete
Maintenance_and_Update_Frequency: None planned
Spatial_Domain:
Description_of_Geographic_Extent:
Locations in Hillsboro, Caloosahatchee, Port Mayaca, Moore Haven, and Kissimme, Florida, USA.
Bounding_Coordinates:
West_Bounding_Coordinate: -82.42
East_Bounding_Coordinate: -80.09
North_Bounding_Coordinate: 34.33
South_Bounding_Coordinate: 26.28
Keywords:
Theme:
Theme_Keyword_Thesaurus: none
Theme_Keyword: Aquifer Storage and Recovery
Theme_Keyword: ASR
Theme_Keyword: Aquifer
Theme_Keyword: Microbial ecology
Theme_Keyword: Metal mobility
Theme_Keyword: Metal toxicity
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: Environment
Theme_Keyword: InlandWaters
Place:
Place_Keyword_Thesaurus: none
Place_Keyword: Florida
Access_Constraints: None
Use_Constraints:
Cite primary author and database when using or publishing these data
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: John Lisle
Contact_Organization: U.S. Geological Survey
Contact_Address:
Address_Type: mailing and physical
Address: 600 Fourth Street South
City: St. Petersburg
State_or_Province: FL
Postal_Code: 33701
Country: USA
Contact_Voice_Telephone: 727-502-8140
Contact_Facsimile_Telephone: 727-502-8181
Contact_Electronic_Mail_Address: jlisle@usgs.gov

Data_Quality_Information:
Logical_Consistency_Report: Not applicable
Completeness_Report: Not applicable
Lineage:
Process_Step:
Process_Description:
Task 1 - Preliminary Characterization of Microbial Ecology of ASR Waters:
1) Characterize the diversity of the microbial communities in native waters associated with CERP ASR wells -
Provides an inventory of the native microbial communities present.
2) Characterize the physiological capacity of the microbial communities in native waters associated with CERP ASR wells -
Provides a measure of which types of nutritional substrates (e.g., proteins, carbohydrates, fatty acids) the native microbes are capable of using for growth and maintenance.
3) Determine the secondary productivity (i.e., bacterial productivity) in the microbial communities in native waters associated with ASR well systems -
Provides rates of metabolism for the native microbe communities.
4) Determine the bacterial abundances and biomass in native waters associated with ASR well systems -
Provides counts and sizes of the native microbe communities.

One primary sample from each exploratory well will be collected and analyzed by USGS as part of the SOW. In addition to the primary samples, one field duplicate sample will be collected at random from one exploratory well for a total of six samples to be collected and analyzed for Task 1.
a) Parameters to meet the objectives outlined above:
(1) Community DNA extraction and diversity assessment using PCR and denaturing gradient gel electrophoresis (DGGE) (Objective 1).
(2) Assessment of community physiological capacities using BioLog EcoPlates (aerobic & facultative anaerobic heterotrophic bacteria) and AN plates (anaerobic bacteria) (Objective 2).
(3) Assessment of secondary bacterial productivity using 3H-leucine as a measurement of protein turnover (Objective 3).
(4) Perform total direct counts using fluorescent stains and microscopy to determine bacterial abundances. These same samples will be used to measure the volume of individual bacterial cells and these data will be used to calculate the bacterial biomass in each sample. All data will be normalized to biomass units so all data will be directly comparable (Objective 4).
Process_Date: 2004
Source_Produced_Citation_Abbreviation:
Lisle, J. (2005). Chracterization of the native microbial communities in Floridan aquifer waters targeted for aquifer storage and recovery (ASR) applications, south central Florida. pp. 29. US Geological Survey. OFR 2005-1036
Process_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: John Lisle
Contact_Organization: U.S. Geological Survey
Contact_Address:
Address_Type: mailing and physical
Address: 600 Fourth Street South
City: St. Petersburg
State_or_Province: FL
Postal_Code: 33701
Country: USA
Contact_Voice_Telephone: 727-502-8140
Contact_Facsimile_Telephone: 727-502-8181
Contact_Electronic_Mail_Address: jlisle@usgs.gov
Process_Step:
Process_Description:
Task 2 - Literature Review of Microbe-Induced Changes in Metal Mobility and Toxicity in ASR Waters
Peer reviewed literature that describes microbial interactions with geologic substrates and how these interactions promote the mobilization and changes in toxicity of metals will be collected and systematically reviewed. Non-peer-reviewed literature will also be included when pertinent to the topic, but will not be the focus of the literature review. Literature sources will be searched and collected using internet search engines (e.g., ScienceDirect, USGS Intranet Library, ISI Web of Science, PubMed, Z39.50) and personal communications and possible visits with federal, state and private organizations that are involved with the types of research that would compile publications and reports pertinent to these topics. The final product will be a reference tool that can be used in the development of subsequent tasks that address this issue. The product of this review will be a synthesis of this body of literature and will focus on the following areas:
a. Background information of how microbes interact with geological material to enhance mobilization and alter toxicity in selected metals.
b. Specific examples of geochemical analyses of groundwaters in south Florida that have shown increases in specific metals over time.
c. Synthesis and interpretation of this body of literature.
d. Identify concepts that are of critical importance to our understanding of how injected waters may alter the mobilization and toxicity of metals in aquifer matrices.
e. Identify concepts that need to be addressed but have not yet been studied.
f. Provide recommendations on what and where problems associated with microbially induced mobilization of metals may occur.
g. Provide a bibliography of pertinent references that can be used as a resource by researchers, planners and administrators.
Process_Date: 2004
Source_Produced_Citation_Abbreviation:
Lisle, J. (2005). Microbially induced changes in metal mobility and toxicity in groundwater: a literature review. US Geological Survey. OFR 2005-1432
Process_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: John Lisle
Contact_Organization: U.S. Geological Survey
Contact_Address:
Address_Type: mailing and physical
Address: 600 Fourth Street South
City: St. Petersburg
State_or_Province: FL
Postal_Code: 33701
Country: USA
Contact_Voice_Telephone: 727-502-8140
Contact_Facsimile_Telephone: 727-502-8181
Contact_Electronic_Mail_Address: jlisle@usgs.gov

Distribution_Information:
Distributor:
Contact_Information:
Contact_Person_Primary:
Contact_Person: Heather S. Henkel
Contact_Organization: U.S. Geological Survey
Contact_Address:
Address_Type: mailing and physical
Address: 600 4th Street South
City: St. Petersburg
State_or_Province: FL
Postal_Code: 33701
Country: USA
Contact_Voice_Telephone: 727-502-8028
Contact_Facsimile_Telephone: 727-502-8182
Contact_Electronic_Mail_Address: hhenkel@usgs.gov
Distribution_Liability: The data have no explicit or implied guarantees.

Metadata_Reference_Information:
Metadata_Date: 20140926
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: Heather S. Henkel
Contact_Organization: U.S. Geological Survey
Contact_Address:
Address_Type: mailing and physical
Address: 600 4th Street South
City: St. Petersburg
State_or_Province: FL
Postal_Code: 33701
Country: USA
Contact_Voice_Telephone: 727-502-8028
Contact_Facsimile_Telephone: 727-502-8182
Contact_Electronic_Mail_Address: sofia-metadata@usgs.gov
Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999
Metadata_Access_Constraints:
The SOFIA staff were unable to contact the principal investigator(s) for this project, and therefore were unable to obtain a review of the information in the metadata record. Any questions about the information should be directed to the Primary Contact Person listed near the bottom of the metadata record. This metadata record may have been copied from the SOFIA website and may not be the most recent version. Please check https://sofia.usgs.gov/metadata to be sure you have the most recent version.

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Last updated: 23 December, 2016 @ 01:49 PM (KP)